Examination of Interchromosomal Interactions in Vegetatively Growing Diploid Schizosaccharomyces pombe Cells by Cre/loxP Site-Specific Recombination
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چکیده
منابع مشابه
Region-specific activators of meiotic recombination in Schizosaccharomyces pombe.
Schizosaccharomyces pombe rec mutants were previously isolated on the basis of their deficiency in meiotic recombination at the ade6 locus. We surveyed their meiotic recombination deficiencies at and between other loci. In rec10 mutants recombinant frequencies in the approximately 2-Mb region surrounding the ade6 locus were reduced 10- to 100-fold, but recombinant frequencies at or between nine...
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In many organisms, meiotic recombination occurs preferentially at a limited number of sites in the genome known as hotspots. In the fission yeast Schizosaccharomyces pombe, simple sequence motifs determine the location of at least some, and possibly most or all, hotspots. Recently, we showed that a large number of different sequences can create hotspots. Among those sequences we identified some...
متن کاملCorrigendum: Site Specific Genetic Incorporation of Azidophenylalanine in Schizosaccharomyces pombe.
“We express our sincere gratitude to the WPH Proteomics Research Technology Platform for the contribution to the mass spectrometry analysis. In addition, we would like to express our thanks to MKB laboratory members for discussion. This work was supported by research funds from National University of Singapore, Wellcome Trust (WT101885MA), The Royal Society, Temasek Life Sciences Laboratory and...
متن کاملSite Specific Genetic Incorporation of Azidophenylalanine in Schizosaccharomyces pombe
The diversity of protein functions is impacted in significant part by the chemical properties of the twenty amino acids, which are used as building blocks for nearly all proteins. The ability to incorporate unnatural amino acids (UAA) into proteins in a site specific manner can vastly expand the repertoire of protein functions and also allows detailed analysis of protein function. In recent yea...
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Mammalian expression systems, due to their capacity in post-translational modification, are preferred systems for biopharmaceutical protein production. Several recombinant protein systems have been introduced to the market, most of which are under clinical development. In spite of significant improvements such as cell line engineering, introducing novel expression methods, gene silencing and pr...
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ژورنال
عنوان ژورنال: Genetics
سال: 2008
ISSN: 1943-2631
DOI: 10.1534/genetics.107.082826